Pre-designed and custom amplicon-based targeted NGS library preparation kits, distributed in the UK & Ireland by Biosecure ID — with full bioinformatics and sample processing support.
CleanPlex® Technology
CleanPlex® is a patented NGS amplicon sequencing technology developed and owned by Paragon Genomics, Inc. It combines an advanced proprietary multiplex PCR primer design algorithm, an exceptionally uniform multiplex PCR amplification chemistry, and an innovative patented background cleaning chemistry — together enabling CleanPlex panels to surpass the performance limits of traditional amplicon-based and hybrid capture-based target enrichment methods.
CleanPlex® is a registered trademark and patented technology of Paragon Genomics, Inc. Distributed in the UK & Ireland exclusively by Biosecure ID Limited. Learn more at paragongenomics.com →
3 hours total · 75 min hands-on · single tube · ≥95% on-target
Non-specific PCR products and primer-dimers are biochemically removed by the proprietary CleanPlex digestion chemistry, ensuring only target sequences are converted into NGS library molecules. More than 20,000 amplicons per primer pool can be multiplexed with >96% mapping rate, >96% on-target rate, and >96% coverage uniformity — maintained across all panel sizes from 15 to 20,000+ amplicons.
CleanPlex's single-tube workflow minimises sample loss to preserve genomic information in challenging samples. High-quality libraries can be generated from as little as 1 ng of DNA, down to 6 pg from a single CTC. Compatible with FFPE tissue, cell-free DNA from liquid biopsies, wastewater, and other degraded sources. Confident variant calling at 1% allele frequency without UMIs.
The entire workflow from purified DNA to sequencing-ready indexed library takes just 3 hours with only 75 minutes hands-on time. A single-tube format minimises sample loss, reduces handling errors, and delivers high reproducibility — Pearson correlation coefficient of 97.26% ± 1.07% across 18 libraries prepared by 3 operators.
Low PCR background, low GC bias, high mapping and on-target rates mean very few reads are wasted on off-target sequences. Compared to Competitor T, CleanPlex requires 60% less sequencing to achieve equivalent data quality — meaning 2.5× more samples can be sequenced per flow cell. Independently validated by Sophia Genetics at AMP 2018.
Duplex Unique Molecular Identifiers (UMIs) incorporated using the same 3-step single-tube protocol enable error correction, PCR duplicate removal, and DNA copy number quantification — pushing sensitivity to 0.1% MAF.
Interested in CleanPlex® technology or want to place a UK/Ireland order?
Pre-Designed Panels
The full Paragon Genomics panel portfolio covers oncology, pharmacogenomics, hereditary cancer, and rare disease. Biosecure ID distributes the complete range across the UK & Ireland, with bioinformatics and sample processing support available for every kit.
Rapid survey of 2,900+ hotspot mutations across 65 cancer genes
Precision medicine made practical — 24 pharmacogenes in one assay
Other CleanPlex® ready-to-use panels available
37 genes · 1,447 amplicons · germline SNV & indel · Boland inversion capable
Full CDS coverage · 237 amplicons · SNV, indel, CNV · 1% somatic LOD · FFPE compatible
All CFTR exons + flanking introns · 65 amplicons · ACMG mutations · 2 ng min input
Cell-free DNA compatible · ultra-sensitive variant detection from liquid biopsy samples
High-resolution HLA typing for transplantation and pharmacogenomics applications
Targeted sequencing for viral and bacterial pathogen detection and strain identification
For the full catalogue visit paragongenomics.com · Biosecure ID holds UK & Ireland distribution rights
Need help choosing the right panel for your application?
Custom Panel Design
CleanPlex® Custom NGS Panels are made-to-order, built using Paragon Genomics' advanced in-silico primer design algorithm and iteratively optimised for maximum performance. Biosecure ID manages the full process — from design brief to delivered kit — with bioinformatics pipeline support included.
Custom assays designed in-silico, optimised, and shipped within ~ 4 weeks of design approval. Wet-lab validation available on request.
From focused single-gene assays to large TMB panels covering megabase-scale genomic regions. New targets can be added without compromising existing panel performance.
Superior primer design covers difficult regions including high-GC, repetitive, and homologous sequences.
Standard chemistry detects 1% MAF. CleanPlex UMI molecular barcoding pushes sensitivity to 0.1% MAF from >30 ng input.
Submit target regions at paragongenomics.com/paragon_designer/ for an instant design coverage report. Biosecure ID can manage this on your behalf.
Design brief through to sequencing data delivery — sample processing, library preparation, and bioinformatics all available as project-based services.
Submit targets via ParagonDesigner™
In-silico design & coverage report
2–4 weeks from approval
BSID bioinformatics support
Bioinformatics Support
Biosecure ID provides end-to-end bioinformatics services for CleanPlex® panels and any NGS workflow — whether you are running our kits, a competitor panel, or whole-genome/exome sequencing. All services are project-based with transparent pricing.
Service tiers
Value-added service workflow